Haem degradation in human haemoglobin in vitro
نویسندگان
چکیده
Human haemoglobin was prepared containing [14C]haem in either the aor the fsubunits. Coupled oxidation of such hybrid haemoglobins with ascorbate and 02 showed that the biliverdin produced by the a-subunits contained approx. 55% aisomer and 45% f-isomer, whereas that produced by the fl-subunits contained approx. 75% a-isomer and 25% P-isomer. Coupled oxidation of isolated aand fl-subunits gave approx. 70% a-isomer, 30% fl-isomer and 78% a-isomer, 22% f-isomer respectively. These results are consistent with calculations of differences in the haem environment in the two subunit types.
منابع مشابه
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تاریخ انتشار 2005